In Vitro Preparation of Actively Maturing Bovine Articular Cartilage Explants for X-Ray Phase Contrast Imaging

• DOI: 10.3791/61607
• Publication Date: 2022-02-01T00:00:14Z
• Authors: Caroline Bissardon; Yadan Zhang; Hélène Rougé Labriet; Sébastien Berujon; Laurent Charlet; Ilyas M. Khan; Emmanuel Brun; Sylvain Bohic
• Keywords: Perichondrium

Understanding the mechanisms that underpin post-natal maturation of articular cartilage is crucial importance for designing next generation tissue engineering strategies and potentially repairing diseased or damaged cartilage. In general, postnatal cartilage, which a wholesale change in collagen structure function to accommodate growth organism, occurs over timescale ranging from months years. Conversely dissolution structural organization also long timescales hallmark degeneration. Our ability study these biological processes detail have been enhanced by findings factors can induce precocious vitro immature The developmental disease related changes occur joint involve bone an co-image tissues would significantly increase our understanding their intertwined roles. simultaneous visualization soft tissue, nowadays challenge overcome conventional preclinical imaging modalities used follow-up. Three-dimensional X-ray Phase-Contrast Imaging methods (PCI) under perpetual developments 20 years due high performance low density objects provide additional information compared imaging. this protocol we procedure experiments biopsy matured data analysis image collected using phase contrast