Microfluidic Synthesis of Microgel Building Blocks for Microporous Annealed Particle Scaffold

• DOI: 10.3791/64119
• Publication Date: 2022-06-17T00:00:46Z
• Authors: Colleen Roosa; Lauren Pruett; Juliana Trujillo; Areli Rodriguez; Blaise Pfaff; Nicholas Cornell; Clare Flanagan; Donald Richieri ...
• Keywords: Microreactor

The microporous annealed particle (MAP) scaffold platform is a subclass of granular hydrogels. It composed an injectable slurry microgels that can form structurally stable with cell-scale porosity in situ following secondary light-based chemical crosslinking step (i.e., annealing). MAP has shown success variety regenerative medicine applications, including dermal wound healing, vocal fold augmentation, and stem cell delivery. This paper describes the methods for synthesis characterization poly(ethylene glycol) (PEG) as building blocks to scaffold. These include custom annealing macromer (MethMAL), determination microgel precursor gelation kinetics, microfluidic device fabrication, generation microgels, purification, basic characterization, sizing annealing. Specifically, high-throughput described herein produce large volumes be used generate scaffolds any desired application, especially field medicine.