Cardiac Loading using Passive Left Atrial Pressurization and Passive Afterload for Graft Assessment

• DOI: 10.3791/66624
• Publication Date: 2024-08-04T00:01:49Z
• Authors: George Olverson; Manuela Lopera Hi...; Maya Bolger-Chen; Emmanuella O. Ajenu; Selena S. Li; Hussein Kharroubi; Bassel Tfayli; Chijioke Chukwudi; Nathan Minie; Joseph Catricala; Allison Pitti; William Michaud; Doug Vincent; David D’Alessandro; S. Alireza Rabi; Shannon N. Tessier; Asishana A. Osho
• Keywords: Afterload; Preload; Contractility

Ex vivo machine perfusion or normothermic is a preservation method that has gained great importance in the transplantation field. Despite immense opportunity for assessment due to beating state of heart, current clinical practice depends on limited metabolic trends graft evaluation. Hemodynamic measurements obtained from left ventricular loading have garnered significant attention within field their potential as objective parameters. In effect, this protocol provides an easy and effective manner incorporating capabilities established Langendorff systems through simple addition extra reservoir. Furthermore, it demonstrates feasibility employing passive atrial pressurization loading, approach that, our knowledge, not been previously demonstrated. This complemented by Windkessel base afterload, which acts compliance chamber maximize myocardial during diastole. Lastly, highlights capability capturing functional metrics cardiac including pulse pressure, contractility, relaxation, uncover deficiencies function after extended periods times (˃6 h).