Molecular subtypes and quantitative analysis of PD-L1 and tumor-associated immune cells in uterine carcinosarcoma

• DOI: 10.3802/jgo.2025.36.e114
• Publication Date: 2025-05-20T08:42:02Z
• Authors: Lili Sun; Xiaozhuo Gao; Zehua Zhao; Yanmei Zhu
• Keywords: Carcinosarcoma

In the present study, molecular subtypes were determined, programmed death-ligand 1 (PD-L1) and tumor-associated immune cells (TAICs) quantitatively detected, their effect on prognosis in uterine carcinosarcoma (UCS) was analyzed. The study included 65 UCS cases. Direct sequencing of POLE exonuclease domain immunohistochemistry mismatch repair (MMR) deficiency proteins p53 used to stratify subtypes. QuPath for quantitative immunohistochemical detection PD-L1 TAICs. chi square test determine association between expression Kaplan-Meier method Cox proportional hazards regression plotting survival analysis. cases, case (1.5%) ultramutated (POLEmut) subtype, 11 cases (16.9%) deficient MMR (dMMR) 32 (49.3%) mutant (p53mut) 21 (32.3%) nonspecific profile (NSMP) subtype. positive density tumor (p=0.022), CD8 stroma (p=0.036), CD163 (p=0.025) significantly associated with patients POLEmut dMMR had a relatively better trend than NSMP p53mut high prognosis; however, showed worse prognosis. could be classified into four M2 macrophages effectively predict UCS.