Ratio of microRNA-122/155 in isoniazid-induced acute liver injury in mice

• DOI: 10.3892/etm.2016.3375
• Publication Date: 2016-05-21T10:37:34Z
• Authors: Lei Song; Zhongrui Zhang; Jinling Zhang; Xuebin Zhu; Lei He; Zhe Shi; Li Gao; Fumin Feng
• Keywords: miR-155

Liver injury is a major hindrance to the treatment of tuberculosis (TB) patients due primary side effects associated with anti‑TB drugs. Several investigations have identified sensitive biomarkers for early diagnosis anti-TB drug-induced liver (ADLI), including use microRNAs (miRNAs/miRs). However, association between miR‑122/155 and ADLI remains unknown. Thus, present study used reverse transcription‑quantitative polymerase chain reaction observe changes in tissue expression levels during course mice. was induced by administration isoniazid (INH), first‑line drug. were quantified at seven time points throughout 1 day (0.25, 0.75, 1.5, 6, 12, 18 24 h) based on pharmacokinetics INH Notably, over timecourse INH‑induced injury, miR‑122 level significantly decreased 0.25 h, which peak concentration INH, compared control group (P<0.05). The change more rapid than that serum aminotransferase miR‑155, increased 0.75 h. In addition, pathological score correlated ratio miR-122/miR-155 (r=‑0.779; P<0.01). conclusion, may be utilized as biomarker ADLI, could contribute following treatment.