MicroRNA‑208a‑3p contributes to connexin40 remolding in human chronic atrial fibrillation

• DOI: 10.3892/etm.2017.5225
• Publication Date: 2017-09-29T08:50:39Z
• Authors: Shanshan Li; Zhiyuan Jiang; Lina Wen; Guirong Feng; Guoqiang Zhong
• Keywords: 0303 health sciences; 03 medical and health sciences; Articles; 3. Good health

Previous studies have demonstrated that connexin40 (Cx40) remolding is involved in atrial fibrillation (AF). GJA5 encoding Cx40 a potential target mRNA of microRNA-208a-3p (miR-208a-3p), as indicated by preliminary bioinformatics analyses. However, the exact effect miR-208a-3p on human chronic AF has remained elusive. The present study role and further investigated expression. A total 19 patients with 18 sinus rhythm (SR) were enrolled. AC16 cell line was treated inhibitor or mimics. right appendage (RAA) tissues measured situ hybridization reverse-transcription quantitative polymerase chain reaction (RT-qPCR). Furthermore, expression RAA cells mimics detected RT-qPCR western blot analysis. luciferase assay performed to confirm whether directly targeted miR-208a-3p. levels significantly increased compared those SR. Conversely, protein decreased lateralization observed AF. led significant upregulation cells, while downregulation. not direct gene findings still suggested may be mediating remolding, represent therapeutic for