Curcumin inhibits the viability, migration and invasion of papillary thyroid cancer cells by regulating the miR‑301a‑3p/STAT3 axis

• DOI: 10.3892/etm.2021.10307
• Publication Date: 2021-06-14T18:28:51Z
• Authors: Ying Liang; Deyu Kong; Yi Zhang; Siqi Li; Yan Li; Liying Dong; Ningxin Zhang; Junfeng Ma
• Keywords: Viability assay; Gentamicin protection assay

Thyroid cancer is one of the most common malignant tumors, and mortality rate associated with thyroid has been increasing annually. Curcumin reported to exert an antitumor effect on papillary (PTC), identification additional mechanisms underlying anticancer curcumin PTC requires further investigation. The present study aimed explore effects viability, migration invasion cells. TPC‑1 cells were incubated different concentrations curcumin, then, cell invasion, wound healing examined by CCK‑8, Transwell assays, respectively. Subsequently, microRNA (miR)‑301a‑3p mimics, miR‑301a‑3p inhibitors signal transducer activator transcription (STAT)3 overexpression vector transfected into cells, migration, reassessed in these Matrix metallopeptidase (MMP)‑2, MMP‑9, epithelial‑mesenchymal transition (EMT)‑related markers, Janus kinase (JAK)/STAT signaling pathway components assessed western blot analysis. significantly inhibited downregulated MMP‑2, MMP‑9 EMT marker expression. Additionally, decreased STAT3 expression upregulating expression, inhibition reversed Furthermore, suppressed JAK/STAT through miR‑301a‑3p/STAT3 axis. data indicated that could inhibit regulating These findings may provide a possible strategy for clinical treatment PTC.