Tristetraprolin regulates phagocytosis through interaction with CD47 in head and neck cancer

• DOI: 10.3892/etm.2022.11478
• Publication Date: 2022-06-29T13:02:57Z
• Authors: Won Lee; Song Kim; Jae An; Tae-Koon Kim; Hee Cha; Hyo Chang; Sang Kim; Seong Kim; Myung Han
• Keywords: CD47; Tristetraprolin; Radioresistance

CD47 is expressed in all human cancer cells, including head and neck cancer, initiates a signaling cascade to inhibit macrophage phagocytosis. However, the mechanism underlying overexpression has not been elucidated radioresistant cancer. The present study demonstrated that decreased Tristetraprolin (TTP) expression induced sustained of using reverse transcription-quantitative PCR western blotting, prevented phagocytosis assay HN31R cell line. Subsequently, TTP transfection, RNA interference, duel-luciferase EMSA, it was revealed transfection enhanced through degradation mRNA by directly binding AREs within 3'UTR. Based on our previous study, methylation-specific blotting DNMT1 overexpressed line epigenetically DMNT1 associated DNA methylation. Overall, these findings provided novel insight into role as biomarker CD47-positive patients.