Crucial role of lncRNA NONHSAG037054.2 and GABPA, and their related functional networks, in ankylosing spondylitis

• DOI: 10.3892/etm.2024.12525
• Publication Date: 2024-03-27T13:18:42Z
• Authors: Penglei Cui; Yanzhuo Zhang; Chao Wang; Bin Xiao; Qianqian Wang; Liang Zhang; Hongchao Li; Chengai Wu; Wei Tian

Long non‑coding RNAs (lncRNAs) have been previously researched in ankylosing spondylitis (AS). Nevertheless, there are few studies of lncRNAs and mRNAs associated with the pathogenesis AS. Differentially expressed (DElncRNAs) (DEmRNAs) between AS normal samples were assessed using R limma package. DOSE packages &lsquo;clusterProfiler&rsquo; exploited for gene enrichment analysis. The functional association proteins protein interactions was STRING database. To investigate important genes subnetworks protein‑protein interaction network, MCODE plug‑in Cytoscape software utilized. mRNA examined via reverse transcription‑quantitative PCR. In total, 152 DEmRNAs 204 DElncRNAs observed samples. A total 68 candidate related to DElncRNA identified. These enriched 30 cellular component terms, 22 molecular functions, 83 biological processes, 9 Kyoto Encyclopedia Genes Genomes, 36 disease ontology pathways. NONHSAG037054.2 most lncRNA genes, <em>GABPA</em> connected NCBI/GenBank accession number not found because it is included NCBI. information can be at website (http://www.noncode.org/show_gene.php?id=NONHSAG037054 https://www.genecards.org/cgi‑bin/carddisp.pl?gene=ACAP2‑IT1). 13 microRNAs (miRNAs) 46 miRNAs <em>GABPA</em>, respectively, found. 173 RNA‑binding both GABPA. addition, downregulated samples, suggesting may diagnostic value conclusion, AS, could serve as a novel factor