Role of albumin in osteoblastic cells: Enhancement of cell proliferation and suppression of alkaline phosphatase activity

• DOI: 10.3892/ijmm.14.6.1077
• Publication Date: 2014-03-10T03:37:31Z
• Authors: Koichi Ishida; Masayoshi Yamaguchi
• Keywords: 0301 basic medicine; Osteoblasts; Cell Count; DNA; Alkaline Phosphatase; Cell Line; Transforming Growth Factor beta1; Mice; 03 medical and health sciences; Gene Expression Regulation; Transforming Growth Factor beta; Albumins; Animals; RNA, Messenger; Insulin-Like Growth Factor I; Protein Kinase Inhibitors; Cell Proliferation

The role of albumin in osteoblastic cells was investigated. Osteoblastic MC3T3-E1 with subconfluent monolayers were cultured for 24 to 72 h a medium without fetal bovine serum (FBS) the presence or absence (0.25, 0.5, 1.0 mg/ml medium). number significantly increased by culture (1.0 mg/ml). effect increasing cell completely abolished PD98049 (10(-7) M), staurosporine dibucaine which is an inhibitor various protein kinases. Also, stimulating on proliferation prevented cycloheximide synthesis, 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB), transcriptional activity. DNA content mg/ml) addition h. Alkaline phosphatase activity cells, participates calcification, decreased expression insulin-like growth factor-I (IGF-I) and transforming factor-beta1 mRNAs using transcription-polymerase chain reaction (RT-PCR) analysis not altered 48 This study demonstrated that had regulation function.