Expression of human aldehyde dehydrogenase-3 associated with hepatocellular carcinoma: promoter regions and nuclear protein factors related to the expression.

• DOI: 10.3892/ijmm.2.3.333
• Publication Date: 2014-03-10T07:33:28Z
• Authors: C Chang; L C Hsu; V Davé; A Yoshida
• Keywords: Carcinoma, Hepatocellular; Liver Neoplasms; Stomach; Alcohol Dehydrogenase; Nuclear Proteins; Electrophoretic Mobility Shift Assay; 3. Good health; DNA-Binding Proteins; Liver; Organ Specificity; Stomach Neoplasms; Cell Line, Tumor; Deoxyribonuclease I; Humans; Promoter Regions, Genetic; Protein Binding

The human ALDH3 gene is constitutively expressed in stomach, lung, esophagus, and cornea, but hardly detectable the normal liver. However, it highly activated hepatocellular carcinoma tissues from approximately 50% of patients. nuclear DNA binding factors exist both ALDH3-positive cancerous liver HepG2 cells, not ALDH3-negative Hep3B cells tissues. South-western blot hybridization showed existence two nuclear-binding protein components, 35 14 kDa, These proteins were found stomach KATO III cells. DNaseI footprint analysis identified protective regions within promoter. first protected region has one putative CCAAT-box Sp1-site. second contains a HiNF-A sequence. findings suggest that high level expression resulted or activation at least reacting to promoter region.