Monosialoteterahexosyl ganglioside induces the differentiation of human umbilical cord-derived mesenchymal stem cells into neuron-like cells

• DOI: 10.3892/ijmm.2015.2307
• Publication Date: 2015-08-10T03:35:57Z
• Authors: CHENGRUI NAN; YUNPENG SHI; ZONGMAO ZHAO; SHUCHENG MA; JIXIANG LIU; DONGDONG YAN; GUOQIANG SONG; HANJIE LIU
• Keywords: CD90

In the present study, human umbilical cord-derived mesenchymal stem cells (hUMSCs) were investigated for their potential to be induced differentiate in vitro into neuron-like by monosialoteterahexosyl ganglioside (GM1). Mononuclear obtained from cords women with full-term pregnancies whose babies delivered cesarean section cultivated and surface antigen expression profiles monitored. Following amplification, divided 5 groups, of which 4 (groups A-D) treated GM1 at doses 50, 100, 150 200 µg/ml, respectively. The control (group E) was vehicle only. ability induce differentiation hUMSCs monitored 6 h. levels microtubule-associated protein-2 (MAP-2), neurofilament protein (NF-H) glial fibrillary acidic (GFAP) measured immunohistochemistry. exposure GM1, first appeared have a diamond or polygonal shape gradually grew long spindle-shaped cells, finally exhibiting radiating swirling pattern. maintained strong proliferative capacity after continuous passage. Flow cytometry revealed that expressed CD73, CD90 CD105 up passage 10, but not CD11b, CD19, CD34, CD45 HLA-DR. Treatment h led appearance oval-shaped cell bodies protruding neurites. These positive MAP-2 NF-H, negative GFAP expression. No changes these markers observed group. Thus, findings study demonstrate effectively induces cells.