Curcumin induces the apoptosis of A549 cells via oxidative stress and MAPK signaling pathways

• DOI: 10.3892/ijmm.2015.2327
• Publication Date: 2015-08-26T02:26:06Z
• Authors: QINGHUA YAO; MIAO LIN; YUQI WANG; YUEBIAO LAI; JINGJING HU; TING FU; LU WANG; SHUYUAN LIN; LIANGLIANG CHEN; YONG GUO
• Keywords: 0301 basic medicine; Oxidative Stress; 03 medical and health sciences; Curcumin; Lung Neoplasms; MAP Kinase Signaling System; Cell Line, Tumor; Humans; Apoptosis; Adenocarcinoma; 3. Good health

Curcumin has been found to exhibit anticancer activity and certain studies have shown that curcumin triggers the apoptosis of human A549 lung adenocarcinoma cells. However, mechanism underlying curcumin‑mediated is not completely understood. The present study was designed investigate effect on induction apoptosis‑related factors in Treatment cells with caused a concentration‑dependent inhibition cell growth an increase apoptosis, as confirmed by THE MTT assay, flow cytometry morphology analysis. Curcumin‑treatment induced loss mitochondrial membrane potential increased cytosolic cytochrome c. Furthermore, curcumin‑induced accompanied changes intracellular oxidative stress‑related enzymes, including decreased reactive oxygen species levels, superoxide dismutase malondialdehyde 4‑hydroxynonenal. In addition, also phosphorylation activation mitogen‑activated protein kinase signaling pathway c‑Jun N‑terminal kinase, p38 extracellular signal-regulated kinase.