Effects of applying amoxicillin in juvenile mice on enamel mineralization and the expression of kallikrein‑related peptidase 4 and tight junction proteins in ameloblasts

• DOI: 10.3892/ijmm.2020.4598
• Publication Date: 2020-05-12T08:15:07Z
• Authors: Jianghong Gao; Xinmei Li; Liping Gao; Haiyan Chen; Bashayer Baras; Xiaojing Liu; Hao Liu; Ayesha Rana; Meili Gao; Jianping Ruan
• Keywords: Amelogenesis

Amoxicillin is a common pediatric drug. However, to the best of our knowledge, role amoxicillin in enamel hypomineralization has not yet been fully elucidated. The aim present study was assess effects on mineralization, morphology ameloblasts, as well expression kallikrein‑related peptidase 4 (KLK4), and tight junction proteins, claudin 1 (CLDN1), (CLDN4) occludin (OCLN), ameloblasts juvenile mice. A total 36 3‑day‑old Kunming mice were randomly divided into three groups. administered 0, 50 or 100 mg/kg by intragastric administration for 19 days. surface calcium (Ca), phosphorous (P) carbon contents mandibular incisors first molars examined scanning electron microscopy energy dispersive X‑ray spectroscopy. Histological changes analyzed hematoxylin eosin staining. KLK4, CLDN1, CLDN4 OCLN levels observed immunohistochemical incidence white patches incisor 100% amoxicillin‑treated greater number defects incisal/occlusal half incisors/molars compared with cervical Following phosphoric‑acid treatment, rod interrod aligned disorderly manner decreased Ca/P ratio molars. More intercellular spaces among maturation KLK4 mature ameloblasts. induced hypomineralization, may be mediated via multiple pathways.