Attenuation of hepatic ischemia‑reperfusion injury by adipose stem cell‑derived exosome treatment via ERK1/2 and GSK‑3β signaling pathways

• DOI: 10.3892/ijmm.2021.5068
• Publication Date: 2021-12-03T11:12:22Z
• Authors: Yaqing Zhang; Yonghua Li; Qilong Wang; Dongyu Zheng; Xue Feng; Wei Zhao; Linlin Cai; Qingqing Zhang; Haitao Xu; Hailong Fu
• Keywords: Aspartate transaminase; Exosome

Exosomes are an emerging therapeutic tool for the treatment of tissue injuries. In present study, protective effect isolated exosomes from adipose‑derived stem cells (ADSCs‑exo) against hepatic ischemia‑reperfusion (I/R) injury was explored. Hepatic I/R achieved by inducing ischemia 60 min followed reperfusion 2 and 6 h. Pre‑treatment with ADSCs‑exo revealed a significant reduction in necrosis apoptosis liver induced injury. Hypoxic oxidative stress managed exosome‑mediated reduced reactive oxygen species increased superoxide dismutase that turn protected mitochondrial damage apoptosis. Reduction inflammatory mediators such as IL‑1β TNF‑α also observed protection hepatocytes evidenced decrease biochemical markers (alanine transaminase, aspartate transaminase lactate dehydrogenase). Exosomal prostaglandin E2 (PGE2)‑mediated ERK1/2 GSK‑3β phosphorylation were to increase Bcl‑2 Bax expression permeability transition pore‑inhibition which may be considered prime mechanism hepatoprotection. conclusion, our results indicated pre‑treatment is effective protecting