Sophoricoside attenuates autoimmune‑mediated liver injury through the regulation of oxidative stress and the NF‑κB signaling pathway

• DOI: 10.3892/ijmm.2023.5281
• Publication Date: 2023-07-17T07:56:03Z
• Authors: Yu Chen; Yu Lei; Han Wang; Lijia Wang; Jiaxin Xu; Shuhui Wang; Meiping Yu; Zhangqi Peng; Fang Xiao; Dean Tian; Mei Liu
• Keywords: Autoimmune Hepatitis

The prevalence of autoimmune hepatitis (AIH) is increasing, yet specific pharmacotherapies remain to be explored. present study aimed investigate the effects sophoricoside (SOP), a bioactive component medical herbs, on AIH and elucidate underlying mechanisms. Bioinformatic approaches were used predict potential targets regulatory mechanisms SOP AIH. evaluated by determining expression levels inflammatory cytokines, histological liver injury hepatic fibrosis in an improved chronic cytochrome P450 2D6 (CYP2D6)‑AIH mouse model concanavalin‑A (ConA)‑induced acute immune‑mediated injury. antioxidant activity was detected vivo vitro experiments. selected signal targeted further confirmed using western blot analysis immunofluorescence staining. results bioinformatic revealed that related oxidative stress NF‑κB gene set. transcription factor family key player controls both innate adaptive immunity. activation signaling pathway often associated with disorders. In animal experiments, attenuated CYP2D6/ConA‑induced AIH, as evidenced significant reduction enzymes serum, cytokine lesions liver. response also significantly inhibited SOP, decrease malondialdehyde, elevations total capacity glutathione peroxidase levels. experiments reduced enhanced nuclear translocation phosphorylated p65 livers mice lipopolysaccharide‑stimulated AML12 cells. On whole, demonstrates protective role which may mediated limiting hepatocytes.