Microdissection based high resolution multicolor banding for all 24 human chromosomes

• DOI: 10.3892/ijmm.9.4.335
• Publication Date: 2014-03-10T07:35:52Z
• Authors: Thomas Liehr; Anita Heller; Heike Starke; Nikolai Rubtsov; Vladimir Trifonov; Kristin Mrasek; Anja Weise; Alma Kuechler; Uwe Claussen
• Keywords: Microdissection

The multicolor-banding (MCB) approach allows the differentiation of chromosome region specific areas at band and sub-band level is based on region-specific microdissection libraries producing changing fluorescence intensity ratios along chromosomes. latter are used to assign different pseudocolors chromosomal regions. We present complete set 138 for entire human genome resulting MCB patterns all chromosomes 450-550 level. In work, creation handling detailed first time. Additionally, unique possibilities technique adjust pseudocolor bands according necessities studied case presented in exemplarity. conclusion, MCB-technique a high resolution alternative other FISH banding approaches suited clarify, which changes appeared complex rearrangements.