Mutation and transcription analyses of the p63 gene in cervical carcinoma.

• DOI: 10.3892/ijo.15.6.1149
• Publication Date: 2014-03-10T07:25:46Z
• Authors: H Nishi; K Isaka; Y Sagawa; S Usuda; A Fujito; H Ito; M Senoo; H Kato; M Takayama
• Keywords: Single-strand conformation polymorphism; Epidermoid carcinoma

Genetic mutation of p53, which monitors DNA damage and operates cellular checkpoints, is a major factor in the development human malignancies. A novel gene p63/p73L/p51, encoding protein with significant homology to p53 p73, was recently identified at 3q27-9. To investigate penetration p63 cervical carcinogenesis, transcription analyses were performed carcinoma. certain isotype called TAp63gamma encodes acidic N-terminus possesses short C-terminus. Using reverse transcriptase-polymerase chain reaction-single strand conformation polymorphism (RT-PCR-SSCP) analysis for TAp63gamma, one found carcinoma cell line SKG-I. However, no mutations causing amino acid substitutions or frameshifts 54 cases examined thought be tumor suppressor gene. While carcinomas tended yield positive signal RT-PCR reaction designed amplify transcripts N-terminus, normal cervix intraepithelial neoplasia (CIN) did not express this transcript. These data suggest that does play an essential role as gene, but expression may speculatively associated growth carcinogenesis.