Alteration of tumor phenotypes of B16 melanoma after genetic remodeling of the ganglioside profile.

Integrins 0303 health sciences Melanoma, Experimental Genes, fos G(M2) Ganglioside Transfection Recombinant Proteins 3. Good health Mice 03 medical and health sciences Phenotype Gangliosides Polypeptide N-acetylgalactosaminyltransferase Tumor Cells, Cultured Animals N-Acetylgalactosaminyltransferases Cell Division
DOI: 10.3892/ijo.17.1.159 Publication Date: 2014-03-10T07:26:02Z
ABSTRACT
Gangliosides have been thought to be tumor markers of various neuroectoderm-derived cancers. However, their roles in malignant phenotypes of cancer cells are not well understood. We have performed the remodeling of ganglioside profiles of mouse melanoma B16 (B78 subline) by introducing GM2/GD2 synthase cDNA, and analyzed the phenotypic changes such as cell morphology, growth, adhesion and c-fos gene expression. Although newly expressed GM2 was clearly detected, GM2-positive transfectant cells showed rather reduced growth rates in vivo and in vitro, lower expression levels of c-fos gene and increased levels of cell adhesion to extracellular matrices compared to control cells. These results suggested that GM2 is involved in the regulation of cell-cell or cell-extracellular matrix interaction, and negatively control cell proliferation.
SUPPLEMENTAL MATERIAL
Coming soon ....
REFERENCES (0)
CITATIONS (0)
EXTERNAL LINKS
PlumX Metrics
RECOMMENDATIONS
FAIR ASSESSMENT
Coming soon ....
JUPYTER LAB
Coming soon ....