Alteration of tumor phenotypes of B16 melanoma after genetic remodeling of the ganglioside profile.

• DOI: 10.3892/ijo.17.1.159
• Publication Date: 2014-03-10T07:26:02Z
• Authors: T Tsurifune; T Ito; X J Li; S Yamashiro; M Okada; T Kanematsu; H Shiku; K Furukawa
• Keywords: Neuroectoderm

Gangliosides have been thought to be tumor markers of various neuroectoderm-derived cancers. However, their roles in malignant phenotypes cancer cells are not well understood. We performed the remodeling ganglioside profiles mouse melanoma B16 (B78 subline) by introducing GM2/GD2 synthase cDNA, and analyzed phenotypic changes such as cell morphology, growth, adhesion c-fos gene expression. Although newly expressed GM2 was clearly detected, GM2-positive transfectant showed rather reduced growth rates vivo vitro, lower expression levels increased extracellular matrices compared control cells. These results suggested that is involved regulation cell-cell or cell-extracellular matrix interaction, negatively proliferation.