A rapid screening assay for antioxidant potential of natural and synthetic agents in vitro

• DOI: 10.3892/ijo.20.5.983
• Publication Date: 2014-03-10T03:27:43Z
• Authors: Praveen Srinivasan; Manicka Vadhanam; Jamal Arif; Ramesh Gupta
• Keywords: Ellagic Acid; Sulforaphane; In vitro toxicology; Pro-oxidant

There are literally thousands of known agents with potential chemopreventive and antioxidant activity; however, the expanding list natural synthetic compounds makes it difficult to test every agent in widely accepted 2-year animal bioassay human clinical trials. Therefore, short-term screening assays needed sort out most efficacious for long-term studies. In present study, identification was explored a Cu2+-mediated Fenton-type reaction, coupled oxidative DNA lesion detection by 32P-postlabeling. Several inhibited formation 8-oxo-2'-deoxyguanosine (8-oxodG), benchmark lesion, but ellagic acid, polyphenol found berries, offered maximal (>80%) inhibition 8-oxodG formation. However, well-known tea polyphenol, epigallocatechin gallate, along silymarin D,L-sulforaphane, exhibited pro-oxidant effect, 50-70% increase induction. general, our results agree reported - activities compounds, rendering this vitro assay be useful determining rapidly cost-effectively.