Knockdown of NANOG enhances chemosensitivity of liver cancer cells to doxorubicin by reducing MDR1 expression

• DOI: 10.3892/ijo.2014.2347
• Publication Date: 2014-03-19T11:40:08Z
• Authors: JIA-JIA ZHOU; XIAO-GENG DENG; XIAO-YU HE; YU ZHOU; MIN YU; WEN-CHAO GAO; BING ZENG; QUAN-BO ZHOU; ZHI-HUA LI; RU-FU CHEN
• Keywords: Homeobox protein NANOG; Rex1; Nanog Homeobox Protein

Multidrug resistance (MDR) is one of the major reasons for failure liver cancer chemotherapy, and its suppression may increase efficacy chemotherapy. NANOG plays a key role in regulation embryonic stem cell self-renewal pluripotency. Recent studies reported that was abnormally expressed several types tumors, indicating related to tumor development. However, correlation between chemoresistance remains uncertain. In this study, RNA interfere technology employed knock down expression HepG2 human cells. We found knockdown siRNA-transfected cells resulted decreased colony formation rate migration compared control addition, were treated with doxorubicin evaluate chemosensitivity doxorubicin. sensitivity increased downregulation expression. The MDR1 at both mRNA protein levels when knocked down. These findings suggest sensitivity, could be used as novel potential therapeutic target reverse multidrug cancer.