Knockdown of hTERT by siRNA inhibits cervical cancer cell growth in vitro and in vivo

• DOI: 10.3892/ijo.2014.2493
• Publication Date: 2014-06-11T07:43:41Z
• Authors: YING-AI SHI; QIANG ZHAO; LI-HONG ZHANG; WEI DU; XUE-YAO WANG; XU HE; SHAN WU; YU-LIN LI
• Keywords: HeLa

Human telomerase reverse transcriptase (hTERT) is the catalytic component of that facilitates tumor cell invasion and proliferation. It has been reported hTERT are significantly upregulated in majority cancers including cervical cancer, thus, downregulation a promising target malignant treatment. We established short interfering RNA (siRNA) targeting hTERT, transfected it into HeLa cells (a cancer line) to investi-gate effect proliferation, apoptosis, migration cells. The results showed siRNA could effectively knock down expression, remarkably suppress activity, invasion, induced apoptosis vitro. In addition, we evaluated whether affects growth nude mice, found dramatically inhibited tumorigenesis mice injected with hTERT. Furthermore, also knockdown was able constitutive phosphorylation Akt, PI3K, which might imply reduction via PI3K/Akt signaling pathway some extent. These suggest suppression expression by inhibits vitro vivo, may provide novel for anticancer gene therapy.