A potential regulatory loop between Lin28B:miR-212 in androgen-independent prostate cancer

• DOI: 10.3892/ijo.2014.2647
• Publication Date: 2014-09-09T10:53:35Z
• Authors: EMMA BORREGO-DIAZ; BENJAMIN C. POWERS; VUGAR AZIZOV; SCOTT LOVELL; RUBEN REYES; BRADLEY CHAPMAN; OSSAMA TAWFIK; DOUGLAS McGREGOR; FRANCISCO J. DIAZ; XINKUN WANG; PETER VAN VELDHUIZEN
• Keywords: DU145

Lin28 is a family of RNA binding proteins and microRNA regulators. Two members this have been identified: Lin28A Lin28B, which are encoded by genes localized in different chromosomes but share high degree sequence identity. The role Lin28B androgen-independent prostate cancer (AIPC) not well understood. expressed all grades prostatic carcinomas cell lines, normal tissue. In study we found that co-localized the nucleus cytoplasm DU145 AIPC. expression protein positively correlated with c-Myc lines silencing also lower protein, downregulation messenger (mRNA) AIPC cells. We hypothesized regul­ates altering intermediate suppressors. Therefore, profile cells was performed after siRNA silencing. Nineteen microRNAs were upregulated eleven downregulated. most miR-212 miR-2278. Prior reports suppressed cancer. then ran TargetScan software to find potential target mRNAs miR-2278, it predicted mRNA as miR-212, or These observations suggest Lin28B:miR-212 may work regulatory loop Furthermore, report predictive 2-fold symmetric model generated superposition structure onto I-TASSER Lin28B. This structural suggests shows unique characteristics. Thus, if bind miRNAs manner similar Lin28A, conformational changes would be necessary prevent steric clashes C-terminal linker regions between CSD ZNF domains.