BANCR contributes to the growth and invasion of melanoma by functioning as a competing endogenous RNA to upregulate Notch2 expression by sponging miR-204

• DOI: 10.3892/ijo.2017.4173
• Publication Date: 2017-10-23T16:10:36Z
• Authors: Bingjie Cai; Yunpeng Zheng; Shanshan Ma; Qu Xing; Xinxin Wang; Bo Yang; Guangwen Yin; Fangxia Guan
• Keywords: Competing Endogenous RNA

BRAF-activated non-coding RNA (BANCR) is a long (lncRNA) that contributes to the initiation and development of many solid tumors, including melanoma. However, BANCR functions downstream mechanisms are largely unknown. In this study, we aim investigate how participates in proliferation migration malignant melanoma elucidate underlying mechanism process. We found expression was low melanocytic nevus human melanocytes but high tissues cell lines. Knockdown inhibited invasion, induced apoptosis. The decreased relative marker proteins further demonstrated inhibitory effect siRNA growth migration. Then, detected downregulation microRNA-204 (miR‑204), suppressor growth, identified miR‑204 direct target neurogenic locus notch homolog protein 2 (Notch2) miR‑204. may promote through inhibition miR‑204, leading activation Notch2 pathway. By tumorigenicity assay BALB/c nude mice, knockdown tumor vivo. Our results suggest BANCR/miR‑204/Notch2 axis mediates progression.