Overexpression of lamin B1 induces mitotic catastrophe in colon cancer LoVo cells and is associated with worse clinical outcomes

• DOI: 10.3892/ijo.2017.4182
• Publication Date: 2017-11-01T10:40:33Z
• Authors: Magdalena Izdebska; Maciej Gagat; Alina Grzanka
• Keywords: Mitotic catastrophe; Nuclear lamina

Lamins are the major components of nuclear lamina and play important roles in many cellular processes. The role lamins cancer development progression is still unclear but it known that reduced expression lamin B1 has been observed colon cancer. Thus, aim present study was to elucidate influence LMNB1 upregulation on cell line after treatment with 5-FU. results indicate, overexpression induced dose-dependent death mainly by mitotic catastrophe pathway. Furthermore, this intermediate protein, lower A/C observed. Moreover, we an increase fluorescence intensity β-catenin decrease cell-cell interaction area, connected inhibition cells migration. We reorganization actin filament β-tubulin, because these cytoskeletal proteins directly or indirectly linked lamins, analyzing publicly available mRNA data show patients characterized survival rates within first 30 months from diagnosis. Summarizing our results, induce only small percentage apoptosis. showed migration promotion contact as a direct indirect regulation β-catenin, A/C, tubulin. However, possible colorectal may be reservoir responsible for faster disease progression, further investigations necessary confirm hypothesis.