Genome-scale analysis of resveratrol-induced gene expression profile in human ovarian cancer cells using a cDNA microarray

• DOI: 10.3892/ijo.22.4.741
• Publication Date: 2014-03-10T07:28:37Z
• Authors: Sang Yang; Jong Kim; Tae Oh; Myung Kim; Sun Lee; Suk Woo; Hyun Cho; Yung Choi; Young Kim; Sun Rha; Hyun Chung; Sung An
• Keywords: Genome*; DNA, Complementary; Time Factors; 610; Antineoplastic Agents; NAD(P)H Dehydrogenase (Quinone)/metabolism; Cell Line; RNA/metabolism; 03 medical and health sciences; 0302 clinical medicine; Ovarian Neoplasms/drug therapy*; Cell Line, Tumor; Stilbenes; NAD(P)H Dehydrogenase (Quinone); Humans; Tumor Suppressor Protein p53/metabolism; Complementary/metabolism*; Neoplastic*/drug effects*; Oligonucleotide Array Sequence Analysis; Cell Nucleus; Ovarian Neoplasms; Tumor; Genome; Reverse Transcriptase Polymerase Chain Reaction; Phytogenic/pharmacology*; DNA; Ovarian Neoplasms/metabolism; 12632063; Antineoplastic Agents, Phytogenic; Up-Regulation; 3. Good health; Gene Expression Regulation, Neoplastic; Stilbenes/pharmacology*; Gene Expression Regulation; Resveratrol; Cell Nucleus/metabolism; RNA; Female; Tumor Suppressor Protein p53

Resveratrol (3,5,4'-trihydroxy-trans-stilbene) is a natural compound found in large quantities, most notably grapes and red wine, which has been shown to have anti-inflammatory, chemopreventive anti-angiogenic effects. We examined whether resveratrol any effect on growth gene expression the human ovarian cancer PA-1 cells. show that inhibits cell induces apoptosis also investigated of changes global during resveratrol-induced inhibition cells using cDNA microarray with 7,448 sequence-verified clones. Out genes screened, 118 were founded be affected their levels by more than 2-fold after 24-h treatment 50 µM resveratrol. at final concentration for 6, 12, 24 48 h patterns analyzed microarray. Clustering modulated three above times points divided into 2 groups. Within these groups, there specific subgroups whose expressions substantially changed specified time points. One highly up-regulated this study was NAD(P)H quinone oxidoreductase 1(NQO-1), recently involved p53 regulation. Although precise roles fluctuate remain elucidated, we hope new view following exposure, as offered study, provides clues mechanism action.