Anthocyanidins induce apoptosis in human promyelocytic leukemia cells: Structure-activity relationship and mechanisms involved

• DOI: 10.3892/ijo.23.3.705
• Publication Date: 2014-03-10T07:29:28Z
• Authors: De-Xing Hou; Takashi Ose; Shigang Lin; Kazuhiro Harazoro; Izumi Imamura; Mayumi Kubo; Takuhiro Uto; Norihiko Terahara; Makoto Yoshimoto; Makoto Fujii
• Keywords: Anthocyanidins; Anthocyanidins; XIAP

Anthocyanidins are the aglycon nucleuses of anthocyanins, which reddish pigments widely spread in colored fruits and vegetables. To investigate their anti-cancer effect, induction apoptosis was tested human promyelocytic leukemia cells (HL-60), is a valid model for testing antileukemic or general antitumoral compounds. Of six anthocyanidins representing aglycons most only those with an ortho-dihydroxyphenyl structure on B-ring induce apoptosis, suggesting that may contribute to apoptosis. Delphinidin, potent inducer, causes time- dose-dependent manner. The efficacious observed at 100 micro M 6 h. Concomitant delphinidin stimulates JNK pathway activation including phosphorylation c-jun gene expression, activates caspase-3. Antioxidants N-acetyl-L-cysteine (NAC) catalase effectively block delphinidin-induced phosphorylation, caspase-3 activation, DNA fragmentation. Moreover, directly cause HL-60 generate intracellular hydrogen peroxide. Thus, trigger apoptotic death program through oxidative stress-involved signaling pathway. by anthocyanins be pivotal mechanism its chemopreventive action against cancer based.