Immunostaining and RT-PCR: different approaches to search for RET rearrangements in patients with papillary thyroid carcinoma

• DOI: 10.3892/ijo.23.4.1025
• Publication Date: 2014-03-10T03:29:14Z
• Authors: Sofia Rebelo; Rita Domingues; Ana Catarino; Evelina Mendonça; Jorge Santos; Luis Sobrinho; Maria Bugalho
• Keywords: Immunostaining; Gene rearrangement; Proto-Oncogene Proteins c-ret

Different techniques of molecular biology have been used to screen for RET rearrangements. More recently, immunohistochemistry has used, assuming that is not expressed in normal thyroid follicular cells. The present study was designed define the prevalence expression patients with papillary carcinoma, by and RT-PCR; search specifically RET/PTC-1; -2; -3 rearrangements using RT-PCR, compare results obtained those RT-PCR. Immunohistochemistry performed a polyclonal antibody against tyrosine kinase domain Ret protein. Screening RET/PTC1-3 RT-PCR specific primers each rearrangement; complementarily, subset cases were tested exon 10/11 detect wild-type RET. Positive staining observed 30 39 (77%) tumours. detected 8 32 (25%) cases. Ten 15 (67%) Two tumours characterised positive immunostaining, absence 5' considered as expressing rearrangement different from RET/PTC-1, -2, or -3. In 3 10 tumours, coexisted rearrangement. does necessarily mean presence it may correspond RET, both. On contrary, without evidence extracellular highly suggestive independently type. Refinement diagnosis depends on primers.