Elevated expression of DNA polymerase κ in human lung cancer is associated with p53 inactivation: Negative regulation of POLK promoter activity by p53

• DOI: 10.3892/ijo.25.1.161
• Publication Date: 2014-03-10T07:29:43Z
• Authors: Yanqing Wang; Mika Seimiya; Kiyoko Kawamura; Ling Yu; Tomoo Ogi; Keizo Takenaga; Tomotane Shishikura; Akira Nakagawara; Shigeru Sakiyama; Masatoshi Tagawa; Jiyang O-Wang
• Keywords: POLK

DNA polymerase κ (POLκ) is a low fidelity translesional implicated in spontaneous and damage-induced mutagenesis. We have previously shown that POLκ was frequently overexpressed human lung cancer tissues as compared with their matched non-tumorous tissue counterpart. In the present study, we found close correlation between elevated expression p53 inactivation tissues. To investigate whether POLK might be regulated by p53, determined transcriptional initiation site of gene examined its promoter activity A549, H358-129, PC-3 cell lines. Wild-type but not mutant (R273H) devoid DNA-binding activity, strongly inhibited these cells. addition, exhibited significantly higher p53−/− murine embryo fibroblasts (MEF) than p53+/− p53+/+ MEF. These results link status suggest loss function may part contribute to observed upregulation cancers.