Constitutive production of proinflammatory cytokines RANTES, MIP-1β and IL-18 characterizes LGL leukemia

• DOI: 10.3892/ijo.26.2.529
• Publication Date: 2014-03-10T07:30:31Z
• Authors: Ravi Kothapalli; Susan Nyland; Irina Kusmartseva; Ratna Bailey; Tylee McKeown; Thomas Loughran
• Keywords: Proinflammatory cytokine

Large granular lymphocyte (LGL) leukemia is a lymphoproliferative disease often associated with autoimmune disorders such as rheumatoid arthritis. High levels of soluble Fas ligand have been implicated in development chronic neutropenia. However, comprehensive analysis constitutive chemokine and lymphokine production LGL has not previously reported. Here, we utilized RNase protection assays enzyme-linked immunosorbent (ELISAs) to address this question. RANTES, IL-8, MIP-1alpha, MIP-1beta, IL-1beta, IL-10, IL-12 p35, IL-18, IFN-gamma IL-1Ra were the cytokine transcripts expressed elevated from RNA peripheral blood mononuclear cells patients. Confirmatory ELISAs indicated that sera patients lesser extent IL-8 IL-1Ra. This pattern upregulation similar seen some infections or diseases, thus characterizing proinflammatory disorder.