hsa_circ_0001508 as a new gene that may promote breast cancer progression via the miR‑505‑3p/HMGB1, VGLL3 axis

• DOI: 10.3892/mco.2024.2808
• Publication Date: 2024-11-28T11:51:49Z
• Authors: Zhe Sun; Wenjing Duan; Jinxian Qian; Liang Chen; Fei Ge

Breast cancer (BC) is a malignant tumor, that damages the physical health of female patients. It is crucial to develop new treatment strategies for BC, as this disease significantly affects the quality of life of women in both developing and developed countries, despite the existence of effective treatment options to reduce mortality. Recently, several researchers have been studying circular RNAs (circRNAs) in BC due to their stability and sponge function. In the present study, a new circRNA, hsa_circ_0001508, was discovered using Gene Chip's prediction. This gene is important because it is novel in BC. However, due to financial constraints, the study was designed as a pilot study for future research. The present study included several common biomedical experimental techniques, such as reverse transcription-quantitative PCR, flow cytometry, cell counting, cell invasion, wound healing assay and western blotting. It also involves the use of DNA microarray (gene chip) and prediction of microRNA and mRNA interactions using bioinformatics tools such as TargetScan. The findings revealed that hsa_circ_0001508 exhibits carcinogenic properties that facilitate the progression of BC. Furthermore, potential binding sites were identified as crucial areas for future investigation.