A simple and practical method that prepares high molecular weight DNA ladders

• DOI: 10.3892/mmr.2012.1061
• Publication Date: 2012-09-04T12:05:49Z
• Authors: JUN-HE ZHANG; RUI YANG; TIAN-YUN WANG; WEI-HUA DONG; FANG WANG; LI WANG
• Keywords: Ethanol precipitation; Multiple displacement amplification; Restriction digest; DNA clamp

The purpose of the current study was to report a simple and practical method prepare high molecular weight (mw) DNA ladders. involves 1,000-4,000-base pairs (bp) fragments being amplified by polymerase chain reaction (PCR), using λ as template. constructed plasmids are digested restriction endonucleases produce 5-, 6-, 8- 10-kb fragments, followed purification precipitation with ethanol, mixed proportionally. 1,000-4,000-bp were successfully generated PCR obtained through digestion plasmids. bands prepared mw ladder clear may aid future biology studies.