Altered melatonin secretion and circadian gene expression with increased proinflammatory cytokine expression in early-stage sepsis patients

• DOI: 10.3892/mmr.2013.1331
• Publication Date: 2013-02-20T10:15:26Z
• Authors: CAI-XIA LI; DONG-DONG LIANG; GUO-HAO XIE; BAO-LI CHENG; QI-XING CHEN; SHUI-JING WU; JUN-LU WANG; WILLIAM CHO; XIANG-MING FANG
• Keywords: Proinflammatory cytokine; Zeitgeber

Inflammatory and immune responses, as well melatonin secretion, are affected by circadian regulation. Abnormal rhythm of release has been reported to be associated with the later stages sepsis; however, its role in early sepsis is unclear. We studied 11 septic non-septic patients our intensive care unit (ICU). Peripheral blood was drawn at 4-h intervals on first day, beginning 2:00 p.m., over a total period 24 h. Plasma levels melatonin, tumor necrosis factor α (TNF-α) interleukin 6 (IL-6) were measured radioimmunoassay or enzyme-linked immunosorbent assay (ELISA). Messenger RNA genes Cry-1 Per-2 analyzed using quantitative real-time PCR. Results show secretion altered sepsis. The acrophase occurred earlier 6:00 compared a.m. ICU patients. Compared group, both expression significantly decreased while TNF-α IL-6 increased [TNF-α, 64.1 (43.6-89.1) vs. 11.4 (10.4-12.5) ng/ml; IL-6, 41.2 (35.7-50.8) 19.1 (16-136.7) median (range), P=0.04]. peak concentrations shown concordance secretion. gene sepsis, which likely led changes pro-inflammatory cytokine release. These findings shed light potential link between progression early-stage