MicroRNA-21 inhibits SMAD7 expression through a target sequence in the 3′ untranslated region and inhibits proliferation of renal tubular epithelial cells

• DOI: 10.3892/mmr.2014.2312
• Publication Date: 2014-06-10T09:06:33Z
• Authors: LI LIN; HUA GAN; HAN ZHANG; WEIXUE TANG; YUE SUN; XUEQIN TANG; DEQUAN KONG; JIAJUN ZHOU; YUXUAN WANG; YANLIN ZHU
• Keywords: 0301 basic medicine; Base Sequence; Genetic Vectors; Lentivirus; Epithelial Cells; Cell Line; Rats; Smad7 Protein; 3. Good health; MicroRNAs; 03 medical and health sciences; HEK293 Cells; Kidney Tubules; Animals; Humans; 3' Untranslated Regions; Sequence Alignment; Cell Proliferation

MicroRNAs (miRNAs) are a class of small non‑coding single‑stranded RNAs that regulate gene expression at the posttranscriptional level. Since identification miRNA, accumulating research has shown their involvement in numerous biological processes, including timing developmental patterning, embryogenesis, cell differentiation, organogenesis, growth control and pathogenesis human diseases. It is estimated >30% genes may be regulated by each miRNA can >100 target mRNAs. The widespread distinct pattern miRNAs normal disease states been extensively investigated context Due to diversity targets, it challenging identify specific elucidate function certain miRNAs. In present study, was confirmed SMAD7 direct miR‑21, overexpression miR‑21 inhibit proliferation rat renal tubular epithelial cells. These findings confirm results previous studies, which have demonstrated regulates protein. However, further investigation required determine whether involved development disease, particularly diabetic nephropathy.