Hepatitis B virus inhibits the expression of CD82 through hypermethylation of its promoter in hepatoma cells

• DOI: 10.3892/mmr.2014.2495
• Publication Date: 2014-08-14T13:44:08Z
• Authors: GUOZHENG YU; YUNTAO BING; WEI LI; LIN XIA; ZHISU LIU
• Keywords: HBx; Metastasis suppressor

The tumor suppressor gene CD82, also known as KAI1, may act a general of metastasis in numerous types cancer. It is hypothesized that downregulation CD82 expression be an important factor the induction hepatocellular carcinoma (HCC), however mechanism for this requires further study. In present study, relative mRNA and protein levels were determined HCC adjacent non‑tumor tissues. association between hepatitis B virus (HBV) was investigated, by quantitative polymerase chain reaction, western blotting, luciferase reporter assays mass spectrometry with matrix‑assisted laser desorption/ionization time‑of‑flight array. shown to suppressed response promoter methylation. Relative downregulated tissues (P<0.05). HBx inhibited activity subsequently levels. Furthermore, it demonstrated HBV could inhibit at transcriptional level, repress through hypermethylation. addition, methyl enzyme inhibitor 5‑aza‑CdR induce level mRNA, observed increase driven promoter. observations study suggest hypermethylation event leading development HCC. Low likely involved progression. hepatoma cells.