Vitamin K2 stimulates Mc3T3‑E1 osteoblast differentiation and mineralization through autophagy induction

• DOI: 10.3892/mmr.2019.10040
• Publication Date: 2019-03-15T08:16:59Z
• Authors: Weiwei Li; Shaokun Zhang; Jie Liu; Yongyi Liu; Qingwei Liang
• Keywords: Viability assay; Vitamin K2

Vitamin K2 likely exerts its protective effects during osteoporosis by promoting osteoblast differentiation and mineralization. However, the precise mechanism remains to be fully elucidated. Autophagy maintains cell homeostasis breaking down eliminating damaged proteins organelles. Increasing evidence in recent years has implicated autophagy development of osteoporosis. The aim present study was verify whether vitamin (VK2) can induce mineralization osteoblasts. In study, MC3T3‑E1 osteoblasts were treated with various doses VK2 (10‑8‑10‑3 M) for 1‑5 days. results revealed no cytotoxicity at concentrations below 10‑5 M, but viability reduced a dose‑dependent manner above M. Furthermore, seeded 6‑well plates complete medium supplemented dexamethasone, β‑glycerophosphate C (VC) osteogenic differentiation. different (10‑5, 10‑6 10‑7 24 h on days 1, 3, 5 7 protocol. It confirmed that promoted using alkaline phosphatase (ALP) alizarin red staining. Using western blotting, immunofluorescence, monodansylcadaverine staining reverse transcription‑quantitative polymerase chain reaction, it observed induced (1 µM) significantly increased ALP activity conversion microtubule associated protein 1 light 3‑α (LC3)II LC3I (P<0.05) every time point. number fluorescent bodies intensity VK2, decreased following treatment 3‑MA+VK2. There an increase mRNA expression levels ALP, osteocalcin (OCN) Runt‑related transcription factor 2 VK2‑treated cells (P<0.01). further association between through inhibitor [3‑methyladenine (3‑MA)]. Osteoblasts 3‑MA exhibited significant inhibition (both P<0.05). addition, osteogenesis VK2+3‑MA group lower compared (P<0.05 both). stimulated MC3T3 promote mineralization, which may potential therapeutic target