Resveratrol affects the expression of uric acid transporter by improving inflammation

• DOI: 10.3892/mmr.2021.12203
• Publication Date: 2021-06-07T15:47:11Z
• Authors: Xuemei Zhang; Qian Nie; Zhimei Zhang; Jing Zhao; Fengxiao Zhang; Chao Wang; Xing Wang; Guangyao Song
• Keywords: Blood Glucose; Inflammation; Male; 0303 health sciences; Body Weight; Caspase 1; Glucose Transport Proteins, Facilitative; NF-kappa B; Membrane Transport Proteins; Articles; Diet, High-Fat; Kidney; MAP Kinase Kinase Kinases; 3. Good health; CARD Signaling Adaptor Proteins; Mice, Inbred C57BL; Disease Models, Animal; 03 medical and health sciences; Myeloid Differentiation Factor 88; NLR Family, Pyrin Domain-Containing 3 Protein; Animals; Cytokines; Insulin Resistance; Chemokine CCL2

Resveratrol (RSV), a polyphenol, non‑flavonoid plant‑derived antitoxin, ameliorates hyperuricemia and kidney inflammation. The present study aimed to establish model of high‑fat diet (HFD)‑induced insulin resistance (IR) determine the specific mechanism RSV improve inflammation reduce uric acid (UA). C57BL/6J mice were fed HFD for 12 weeks their glucose tolerance was evaluated by intraperitoneal testing. then administered 6 weeks, blood samples collected. Serum UA concentrations determined using ELISA kits. Hematoxylin eosin, periodic acid‑Schiff Masson staining performed observe pathological changes kidney, electron microscopy used in ultrastructure. renal interleukin (IL)‑6, IL‑18, IL‑1β tumor necrosis factor‑α (TNF‑α) measured kits, western blotting protein expression levels various indicators. significantly ameliorated HFD‑induced IR reduced levels. Long‑term can lead lipid deposition, glycogen accumulation, inflammatory damage fibrotic mice. This leads significant increase transport‑related proteins, an reabsorption Notably, intervention able reverse this process. effect may be achieved inhibiting NOD‑like receptor family, pyrin domain‑containing 3 (NLRP3) inflammasome Toll‑like 4 (TLR4)/myeloid differentiation factor 88/nuclear factor‑κB signaling pathway. In conclusion, through TLR4 NLRP3 pathways, transporter proteins insulin‑resistant mice, thereby reducing