Alteration of relative telomere length and telomerase reverse transcriptase expression in the granulosa cells of women during aging and assessment of in vitro fertilization outcomes

• DOI: 10.3892/mmr.2023.13093
• Publication Date: 2023-09-18T08:58:52Z
• Authors: Yan Hao; Mingrong Lv; Jing Peng; Dan Kuang; Zhihua Zhang; Zhou Zhang; Tingting Wang; Bo Yang; Zhaolian Wei; Ping Zhou; Zhiguo Zhang; Yunxia Cao
• Keywords: Adult; Male; Aging; Granulosa Cells; Articles; Fertilization in Vitro; Aneuploidy; Pregnancy; Semen; Humans; Female; Telomerase; Aged; Retrospective Studies

Telomere attrition plays a critical role in the reproductive aging process humans. length (TL) is typically regulated by telomerase, main component of which telomerase reverse transcriptase (TERT). The aim present study was to investigate changes relative TL (RTL) and TERT expression granulosa cells (GCs) during its association with reproduction. Clinical data on frozen‑thawed embryo transfer cycles older (>35 year old) younger (≤35 women from single center over 3‑year period were retrospectively analyzed. Preimplantation genetic testing for chromosome aneuploidies same also Following analysis data, several biological characteristics senescent GCs explored. In addition, total 160 who undergoing their first fresh cycle vitro fertilization (IVF) or intracytoplasmic sperm injection included study. collected all participants. RTL investigated using quantitative PCR western blotting. associations IVF outcomes assessed. clinical demonstrated that pregnancy live birth rates aged >35 years ~20% lower than those ≤35 years, number embryos aneuploidy 7‑fold without euploidy age group. An aging‑induced change follicle stimulating hormone receptor observed. A shorter increased detected women. significant inverse correlation between levels oocyte yield identified. However, no blastocyst formation rate probability detected. It may be concluded alterations are potential determinants ovarian aging. appears biomarker prediction response, provides novel strategy assessment female fertility.