An intricate regulatory circuit between FLI1 and GATA1/GATA2/LDB1/ERG dictates erythroid vs. megakaryocytic differentiation

• DOI: 10.3892/mmr.2024.13231
• Publication Date: 2024-04-26T11:36:06Z
• Authors: Chunlin Wang; Maoting Hu; Kunlin Yu; Wuling Liu; Anling Hu; Yi Kuang; Lei Huang; Babu Gajendran; Eldad Zacksenhaus; Xiao Xiao; Yaacov Ben‑David
• Keywords: GATA1; GATA2; Chromatin immunoprecipitation

During hematopoiesis, megakaryocytic erythroid progenitors (MEPs) differentiate into or lineages in response to specific transcriptional factors, yet the regulatory mechanism remains be elucidated. Using MEP‑like cell line HEL western blotting, RT‑qPCR, lentivirus‑mediated downregulation, flow cytometry as well chromatin immunoprecipitation (ChIp) assay demonstrated that E26 transformation‑specific (ETS) transcription factor friend leukemia integration 1 (Fli‑1) inhibits differentiation. The present study using these methods showed while FLI1‑mediated downregulation of GATA binding protein (GATA1) suppresses erythropoiesis, its direct induction GATA2 promotes GATA1 is also involved differentiation through regulation GATA2. By contrast FLI1, ETS member erythroblast transformation‑specific‑related gene (