Redox status expressed as GSH:GSSG ratio as a marker for oxidative stress in paediatric tumour patients

• DOI: 10.3892/ol.2012.931
• Publication Date: 2012-09-21T11:44:14Z
• Authors: ONDREJ ZITKA; SYLVIE SKALICKOVA; JAROMIR GUMULEC; MICHAL MASARIK; VOJTECH ADAM; JAROMIR HUBALEK; LIBUSE TRNKOVA; JARMILA KRUSEOVA; TOMAS ECKSCHLAGER; RENE KIZEK
• Keywords: Retinoblastoma

Oxidative stress causes profound alterations of various biological structures, including cellular membranes, lipids, proteins and nucleic acids, it is involved in numerous malignancies. Reduced glutathione (GSH) considered to be one the most important scavengers reactive oxygen species (ROS), its ratio with oxidised (GSSG) may used as a marker oxidative stress. The main aim this study was determine GSH:GSSG blood serum paediatric cancer patients use potential whole procedure optimised recoveries for both substances were greater than 80% under conditions. We analysed group (n=116) types cancer, neuroblastoma, anaplastic ependymoma, germ cell tumour, genital tract lymphadenopathy, rhabdomyosarcoma, nephroblastoma, Ewing's sarcoma, osteosarcoma, Hodgkin's lymphoma, medulloblastoma retinoblastoma. simultaneously determined levels reduced glutathione, thus, patients. highest observed retinoblastoma lowest ependymoma. able distinguish between diagnoses based on results obtained ratio.