Fusobacteria alterations are associated with colorectal cancer liver metastasis and a poor prognosis

• DOI: 10.3892/ol.2024.14368
• Publication Date: 2024-03-26T12:28:11Z
• Authors: Min Jin; Qilin Fan; Fumei Shang; Tao Zhang; Shuji Ogino; Hongli Liu
• Keywords: Fusobacteria

Liver metastasis is a major cause of mortality in patients with advanced stages colorectal cancer (CRC). The gut microbiota has been demonstrated to influence the progression liver diseases, potentially providing novel perspectives for diagnosis, treatment and research. However, microbial characteristics CRC (LM) no (NLM) have not yet fully established. In present study, high‑throughput 16S RNA sequencing technology was employed, order examine richness composition LM or NLM. A discovery cohort (cohort 2; LM=18; NLM=36) validation 3; LM=13; NLM=41) were established using fresh feces. addition, primary carcinoma tissue samples also analyzed (LM=8 NLM=10) as supplementary 1). findings study indicated that intestinal diversity increased group compared NLM group. significant difference observed species between two cohorts different samples, dominant phyla consistent, but varied at lower taxonomic levels. Phylum Fusobacteria presented consistent enrichment both cohorts. Furthermore, application random forest model receiver operator characteristic curve analysis, identified potential biomarker LM. Moreover, poor prognosis factor survival. Importantly, reconfirmed cohort. On whole, exhibit distinct characteristics. detection thus use predicting CRC.