MicroRNA-195-5p is a potential diagnostic and therapeutic target for breast cancer

• DOI: 10.3892/or.2014.2971
• Publication Date: 2014-01-08T13:54:27Z
• Authors: QIFENG LUO; CHUANKUI WEI; XIAOYU LI; JIA LI; LEI CHEN; YIXIANG HUANG; HONGMING SONG; DENGFENG LI; LIN FANG
• Keywords: Cyclin E1

MicroRNAs (miRNAs) are a class of highly conserved, small endogenous single-strand non-coding RNAs. They aberrantly expressed in the circulation and tissue patients with cancer. Therefore, it has been suggested that they may act as key regulators carcinogenesis. The aim present study was to examine expression level miR-195-5p human breast cancer its potential role measured 40 specimens adjacent normal tissues by quantitative polymerase chain reaction (qPCR). Next, explore function miR-195-5p, we used MDA-MB-231 cells carried out MTT, colony formation, Transwell chamber migration cell cycle assays. dual-luciferase reporter assay also performed determine putative targets which were validated using qPCR western blot We found significantly decreased when compared (P<0.05). Overexpression inhibited proliferation, reduced suppressed caused an accumulation G1 phase cycle. In 3'-untranslated region (3'-UTR) cyclin E1 (CCNE1), two target sites bind suggesting CCNE1 is direct miR-195-5p. Furthermore, through assays showed overexpression mRNA protein levels, respectively. Our suggests tumor suppressor targeting this miRNA provide novel strategy for diagnosis treatment lethal disease.