RNAi-mediated RPL34 knockdown suppresses the growth of human gastric cancer cells

• DOI: 10.3892/or.2015.4219
• Publication Date: 2015-08-21T07:39:01Z
• Authors: HUI LIU; SHAOHUA LIANG; XI YANG; ZHAONING JI; WENYING ZHAO; XIAOBING YE; JING RUI
• Keywords: Ribosomal Proteins; 0301 basic medicine; Gene Expression; Apoptosis; Articles; Cell Cycle Checkpoints; 3. Good health; 03 medical and health sciences; Stomach Neoplasms; Cell Line, Tumor; Gene Knockdown Techniques; Humans; RNA Interference; Cell Proliferation

An increasing body of evidence suggests that ribosomal proteins may have ribosome-independent functions and may be involved in various physiological and pathological processes. To examine the role of ribosomal protein L34 (RPL34) in cancer transformation, we assessed its expression in gastric cancer cell lines and found it highly expressed. We further used lentivirus-mediated small interfering RNAs (siRNAs) to knockdown RPL34 expression in the human gastric cancer cell line SGC-7901. RNA interference (RNAi)-mediated inhibition of RPL34 expression in SGC-7901 cells significantly suppressed cell proliferation, increased apoptosis and arrested cells in the S phase. The results of the present study suggest that RPL34 plays a critical role in cell proliferation, cell cycle distribution and apoptosis of human malignant gastric cells.