miR-30a radiosensitizes non-small cell lung cancer by targeting ATF1 that is involved in the phosphorylation of ATM

• DOI: 10.3892/or.2017.5448
• Publication Date: 2017-02-14T12:10:28Z
• Authors: Yuyan Guo; Wenze Sun; Tuotuo Gong; Yanlan Chai; Juan Wang; Beina Hui; Yi Li; Liping Song; Ying Gao
• Keywords: Radiosensitivity

Increasing number of studies report that microRNAs play important roles in radiosensitization. miR-30a has been proved to perform many functions the development and treatment cancer, it is downregulated non-small cell lung cancer (NSCLC) tissues cells. This study was conducted understand if plays a role radiosensitivity NSCLC Radiosensitivity examed by colony survival assay tumor volume changing vitro vivo, respectively. Bioinformatic analysis luciferase reporter assays were used distinguish candidate target miR-30a. qRT-PCR western blotting carried out detect relative expression mRNAs proteins. Cell cycle apoptosis determined flow cytometry. Our results illustrated could increase NSCLC, especially A549 line. In vivo experiment also showed potential radiosensitizing possibility Further exploration validated directly targ­eting activating transcription factor 1 (ATF1). studying ataxia-telangiectasia mutated (ATM) associated effects on radiosensitivity, we found reduce radiation induced G2/M arrest may affect apoptosis. Together, our demonstrated modulate through reducing function ATF1 phosphorylation ATM have therapeutic value.