CDCA5 promotes the progression of prostate cancer by affecting the ERK signalling pathway

• DOI: 10.3892/or.2021.7920
• Publication Date: 2021-01-04T07:50:09Z
• Authors: Junpeng Ji; Tianyu Shen; Yang Li; Yixi Liu; Zhiqun Shang; Yuanjie Niu
• Keywords: Male; 0301 basic medicine; MAP Kinase Signaling System; Cell Cycle; Prostate; Prostatic Neoplasms; Cell Cycle Proteins; Articles; Prognosis; 3. Good health; Gene Expression Regulation, Neoplastic; Mice; 03 medical and health sciences; Cell Movement; Cell Line, Tumor; Animals; Humans; Adaptor Proteins, Signal Transducing; Aged; Cell Proliferation

Cell division cycle-associated 5 (CDCA5) can regulate cell cycle-related proteins to promote the proliferation of cancer cells. The purpose present study was investigate expression level CDCA5 in prostate (PCa) and its effect on PCa progression. signalling pathway by which functions through also attempted elucidate. Clinical specimens patients were collected from Second Hospital Tianjin Medical University. tissues paracancerous detected RT-qPCR analysis IHC. relationship between survival rate analysed using TCGA database. Two stable lines (C4-2 PC-3) with knockdown established, effects MTT colony formation assays. Flow cytometry performed detect cycle, western blot used determine changes ERK phosphorylation levels after knockdown. tumour growth assessed a mouse xenograft model. results revealed that mRNA protein significantly higher than tissues. High associated prognosis PCa. reduced number cells mitoses inhibited their vitro vivo. When knocked down, reduced. Collectively, upregulated affected Decreased inhibiting pathway. Thus, may be potential therapeutic target for