Downregulation of triggering receptor expressed on myeloid cells 1 inhibits invasion and migration of liver cancer cells by mediating macrophage polarization

• DOI: 10.3892/or.2021.7988
• Publication Date: 2021-02-19T11:15:52Z
• Authors: Minmin Chen; Ruimin Lai; Xiaoyu Lin; Wei Chen; Huizhen Wu; Qi Zheng
• Keywords: Macrophage polarization

Triggering receptor expressed on myeloid cells‑1 (TREM1) is a cell‑surface protein tumor‑associated macrophages (TAMs), the predominant inflammatory cells in tumor microenvironment; however, mechanisms for influence of TREM1 TAM polarization during liver cancer progression have not been investigated. In present study, 20 patients diagnosed with hepatocellular carcinoma (HCC) who underwent surgery were enrolled, and expression M1/M2 M2 was assessed by immunohistochemical staining. Human leukemia monocytic (THP‑1) differentiated into using phorbol 12‑myristate 13‑acetate, IL‑4 IL‑13. A specific short hairpin RNA used to knockdown expression. To investigate effects downregulation migration invasion cells, HepG2 MHCC97H cell lines co‑cultured conditioned media. Reverse transcription‑quantitative PCR western blot analyses detect M1 macrophage marker The levels proteins PI3K/AKT/mTOR signaling pathway analyzed blotting, revealing that HCC tissues significantly elevated compared adjacent normal tissues, highly membranes tissues. results demonstrated shifted towards an phenotype, as defined higher M1‑associated markers decreased M2‑associated markers. addition, suppressed cells. Furthermore, TREM1‑knockdown inhibited activation macrophages. conclusion, phenotype via inhibiting PI3K/AKT signaling. when this blocked. findings suggest novel potential therapeutic target management.