Yin Yang 1 regulates ITGAV and ITGB1, contributing to improved prognosis of colorectal cancer

• DOI: 10.3892/or.2022.8298
• Publication Date: 2022-03-09T08:47:02Z
• Authors: Nami Sato; Nozomu Sakai; Katsunori Furukawa; Tsukasa Takayashiki; Satoshi Kuboki; Shigetsugu Takano; Gaku Ohira; Hisahiro Matsubara; Masayuki Ohtsuka
• Keywords: YY1

Yin Yang 1 (YY1) is a multifunctional transcription factor with critical roles in carcinogenesis and metastasis. However, its biological role clinical impact colorectal cancer (CRC) remain unclear. In the present study, function underlying molecular mechanisms of YY1 CRC progression were investigated. The immunohistochemistry (IHC) 143 tissues revealed significant correlation low expression aggressive clinicopathological features, increased metastasis recurrence poor patient survival. Multivariate analysis identified as an independent prognostic factor. Subsequently, IHC 66 paired primary tumor liver that was significantly associated multiple metastases, major hepatectomy, extrahepatic prognosis. vitro experiments knockdown promoted migration invasion cells. To examine downstream genes YY1, cDNA microarray assay conducted differentially expressed between YY1‑knockdown control cells compared. Integrin alpha V (ITGAV) integrin beta (ITGB1) upregulated hub using gene enrichment protein‑protein interaction analyses. Western blotting confirmed to be negatively correlated ITGAV ITGB1 expression. summary, it tumor‑suppressor CRC, contributes survival patients CRC. Low prognosis metastases. suppressed ITGB1, this transcriptional regulation may lead suppression cell invasion.