OTX1 promotes tumorigenesis and progression of cervical cancer by regulating the Wnt signaling pathway

• DOI: 10.3892/or.2022.8419
• Publication Date: 2022-09-29T08:18:22Z
• Authors: Limin Zhou; Hongying Li; Dunlan Zhang; Lu Chen; Hong Dong; Yuqin Yuan; Tinghui Wang
• Keywords: MTT assay

Cervical cancer is a common malignant tumor in females. Orthodenticle homolog 1 (OTX1) serves key role the occurrence and progression of tumors. The present study aimed to investigate potential mechanism OTX1 cervical cancer. expression was analyzed by western blotting, reverse transcription‑quantitative PCR immunohistochemistry. MTT assay performed assess cell viability. EdU colony formation were used measure proliferation. Wound healing Transwell assays migration invasion. Western blot for assessment protein expression. Gene set enrichment analysis (GSEA) analyze signaling pathways regulated OTX1. Co‑Immunoprecipitation confirm interaction between Wnt9b. In tissue cells, significantly upregulated. overexpression promoted proliferation, invasion cells. silencing decreased GSEA showed that activated Wnt pathway. inhibited increased levels adenomatous polyposis coli (APC), glycogen synthase kinase (GSK)‑3β axis inhibition (AXIN)2 Wnt9b β‑catenin. APC, GSK‑3β AXIN2 However, XAV939 (a inhibitor) β‑catenin partly eliminated effect on activating