The gut microbiota diversity of five Orthoptera (Insecta, Polyneoptera) insects determined by DNA metabarcoding

• DOI: 10.3897/bdj.11.e98162
• Publication Date: 2023-03-15T08:30:15Z
• Authors: Yantong Liu; Lina Zhao; Zhongying Qiu; Hao Yuan
• Keywords: Grasshopper; Pantoea

Most orthopteran insects are phytophagous and some important pests in agriculture forests. Many intestinal microflora of Orthoptera have been reported, primarily from Acridoidea there few reports other taxa. In this study, we collected 15 individuals representing five species ( Ruspolia lineosa , Tetrix japonica Erianthus versicolor Gryllotalpa orientalis Teleogryllus emma ) belonging to superfamilies (Tettigonioidea, Tetrigoidea, Eumastacoidea, Gryllotalpoidea Grylloidea) characterise compare the gut microbiota with greater taxonomic width by performing sequencing analysis 16S rRNA V4 region material. A total 606,053 high-quality sequences 3,105 OTUs were acquired samples 24 phyla, 48 classes, 69 orders, 133 families 219 genera. Firmicutes bacteria most abundant followed Bacteroidetes, Cyanobacteria, Actinobacteria Acidobacteria. At genus level, Serratia Citrobacter Wolbachia Lactobacillus Parabacteroides predominant genera R. T. E. G. respectively. Both Principal Coordinates Analysis (PCoA) heatmap results revealed significant differences bacterial community composition across species. Additionally, alpha diversity indicated richness was significantly different amongst