Low-copy nuclear gene and McGISH resolves polyploid history of Eleusine coracana and morphological character evolution in Eleusine

• DOI: 10.3906/bot-1305-12
• Publication Date: 2013-12-12T07:55:32Z
• Authors: Qing LIU; Bin JIANG; Jun WEN; Paul Michael PETE...
• Keywords: Eleusine; Polyploid; Eleusine indica

Eleusine coracana (L.) Gaertn. (finger millet) is the third most important cereal crop in semiarid regions of world, but degree relatedness finger millet with other species genus unverified. The study morphological character evolution has lagged behind due to lack relevant research. Polyploidy history was analyzed using waxy sequences together multicolor genomic situ hybridization (McGISH). In phylogenetic tree, 2 homoeologous loci were isolated from 3 tetraploids: coracana, E. africana Kenn.-O'Byrne, and kigeziensis S.M.Phillips. grouped indica tristachya (Lam.) Lam. W-A1 clade (A-type sequences). W-B1 W-B2 clades (B-type sequences) composed africana-E. jaegeri Pilg.-E. subclades, respectively. probes produced stronger signals relative intensities across A-genome chromosomes than did probes. tree McGISH evidence support (or its extinct sister or ancestor) as primary secondary parents for millet, likely scenario that B-genome donor extinct. Five characters found be homoplasious by optimization on gene tree.